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Proceedings Paper

Advances in the development of lanthanide macrocyclic complexes as luminescent biomarkers
Author(s): Adedoyin M. Adeyiga; Patrick M. Harlow; Lidia M. Vallarino; Robert C. Leif
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Paper Abstract

The development of peripherally substituted europium(III)-macrocycles suitable as luminescent bio-markers was continued in three related areas. (1) Protocols were established for the coupling of NCS-substituted Eu-macrocycles to proteins and for the mounting on microscope slides of particles labeled with luminescent Eu-macrocycles. The emission/excitation spectra of the dried, slide-mounted particles were investigated. (2) A procedure was developed for the synthesis of lanthanide-macrocycles having a single peripheral functionality. The structure and properties of the mono-functionalized macrocyclic complexes were established. (3) A study was undertaken to explore whether the emission intensity of the Eu-macrocycles can be increased by energy transfer from yttrium(III) complexes. Preliminary results have shown that a considerable luminescence enhancement can be achieved by this method. The results obtained in these three areas are evaluated in the light of the research reported by other investigators.

Paper Details

Date Published: 10 May 1996
PDF: 9 pages
Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); doi: 10.1117/12.239509
Show Author Affiliations
Adedoyin M. Adeyiga, Virginia Commonwealth Univ. (United States)
Patrick M. Harlow, Virginia Commonwealth Univ. (United States)
Lidia M. Vallarino, Virginia Commonwealth Univ. (United States)
Robert C. Leif, Newport Instruments (United States)

Published in SPIE Proceedings Vol. 2678:
Optical Diagnostics of Living Cells and Biofluids
Daniel L. Farkas; Robert C. Leif; Alexander V. Priezzhev; Toshimitsu Asakura; Bruce J. Tromberg, Editor(s)

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