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Proceedings Paper

High-speed electronics for the detection of time-resolved fluorescence in a continuous flow system
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Paper Abstract

In single molecule detection by laser induced fluorescence, a main problem is the low signal to noise ratio due to scattering of the exciting laser light. One common approach to solve this problem is the application of time resolved techniques. Here we present a high speed electronic (based on a pair of PC cards) specially suited for detection of TCSPC curves in a continuous flow system. The whole system works on two different time scales: a millisecond time scale (every millisecond a complete TCSPC curve is measured and stored) and a picosecond time scale (showing the fluorescence decay). The technique present here is of particular interest for applications such as fast DNA sequencing, where a distinction between the different bases solely by the decay times of the attached fluorescence labels is conceivable.

Paper Details

Date Published: 1 April 1996
PDF: 6 pages
Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); doi: 10.1117/12.237635
Show Author Affiliations
Rainer Erdmann, BiosQuant GmbH (Germany)
Uwe Ortmann, BiosQuant GmbH (Germany)
Joerg Enderlein, BiosQuant GmbH (Germany)
Wolfgang Becker, Becker & Hickl GmbH (Germany)
Michael Wahl, BiosQuant GmbH (Germany)
Edgar O. Klose, BiosQuant GmbH (Germany)

Published in SPIE Proceedings Vol. 2680:
Ultrasensitive Biochemical Diagnostics
Gerald E. Cohn; Steven A. Soper; C. H. Winston Chen, Editor(s)

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