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Proceedings Paper

Matrix-assisted laser desorption analysis of corticotropin-releasing factor-binding protein and activin by means of immunoprecipitation
Author(s): A. Grey Craig; Steven W. Sutton; Joan Vaughan; Wolfgang H. Fischer
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Paper Abstract

A sensitive immunoprecipitation procedure suitable for matrix assisted laser desorption mass spectrometry (MALD-MS) has been developed for the analysis of proteins. The ultimate goal of this work is to develop a method suitable for analysis of proteins from biological fluids such as plasma, serum and expression media at physiologically relevant concentrations. Proteins are specifically precipitated from complex mixtures by immunoprecipitation with antibodies directed against portions of the protein of interest and the immunoprecipitates are analyzed by MALD-MS. Here we show that the method is applicable to two proteins, the 35 kDa glycoprotein, corticotropin releasing factor-binding protein (CRF-BP) and the 26 kDa homodimeric protein, activin. In order to elucidate the constraints for achieving our ultimate goal we report MALD-MS experiments and parallel analyses with gel electrophoresis and immunostaining. Using this MALD-MS immunoprecipitation procedure we have been able to successfully analyze samples of CRF-BP or activin in the presence or absence of serum.

Paper Details

Date Published: 1 April 1996
PDF: 12 pages
Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); doi: 10.1117/12.237633
Show Author Affiliations
A. Grey Craig, The Salk Institute (United States)
Steven W. Sutton, The Salk Institute (United States)
Joan Vaughan, The Salk Institute (United States)
Wolfgang H. Fischer, The Salk Institute (United States)

Published in SPIE Proceedings Vol. 2680:
Ultrasensitive Biochemical Diagnostics
Gerald E. Cohn; Steven A. Soper; C. H. Winston Chen, Editor(s)

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