Fluorescence imaging in situ may provide highly specific identification of cell types and altered metabolic activity near the surface of tissue. Most approaches to developing the necessary analytical framework for quantitative 3-D use are based on numerical solutions of some form of transport equation. These are highly computer-intensive and can only be carried out for specified parameters. We apply a random walk model for photon migration which enables us to find an exact expression for the frequency-dependent fluorescent signal emitted from the site of a single fluorophore. Our general expression allows for broad variation of the degree of absorptivity, and is potentially important in providing a basis for the development of fluorescence image reconstruction algorithms.

Date Published: 5 April 1996
PDF: 8 pages
Proc. SPIE 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy, (5 April 1996); doi: 10.1117/12.237577

Published in SPIE Proceedings Vol. 2679:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy
Robert R. Alfano; Abraham Katzir, Editor(s)