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Proceedings Paper

Glial reactions to argon laser photocoagulation injury in rabbit and rat retinas
Author(s): Martin F. Humphrey; Yi Chu; Claudia Sharp; Stephen Moore; Krishna Mann; Piroska Rakoczy; Ian J. Constable
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Paper Abstract

Argon laser photocoagulation is a standard and effective clinical technique for a variety of disease conditions. However there is evidence that coagulation produces more widespread alterations in the retina than the local scarring at the injury site. For example, in diabetic retinopathy multiple photocoagulations in the retinal periphery can control blood vessel growth in the central retina. Therefore we have studied the changes in retinal glial cells following photocoagulation using immunocytochemical techniques with an emphasis on the spread of cellular reactions by using whole, flatmounted retinal preparations. Muller glial cells do not normally express the cytoskeletal protein GFAP (glial fibrillary acidic protein) but do so after a variety of injuries. We found that there is a very widespread expression of GFAP by Muller cells even after very focal coagulations and that this persists for 1 - 1.5 months after coagulation. The microglial cells are primed to react to injury and can release very powerful effector molecules and we therefore also examined the microglial reaction to see whether it correlated with the Muller cell reaction. However, we found that the microglial response, in terms of anatomical changes, was very focally confined to regions of direct cellular injury. We also examined MHC II expression to see whether microglia expressed this activity related protein without anatomical changes but we found no evidence of wide spread changes. In summary we find that inflammatory reactions are very localized after coagulation but the macroglial changes are more widespread and therefore the distant effects of photocoagulation may be more related to macroglial reactions.

Paper Details

Date Published: 1 April 1996
PDF: 9 pages
Proc. SPIE 2674, Laser-Inflicted Eye Injuries: Epidemiology, Prevention, and Treatment, (1 April 1996); doi: 10.1117/12.237498
Show Author Affiliations
Martin F. Humphrey, WARP Research Ctr. (Australia) and Univ. Magdeburg (Germany)
Yi Chu, WARP Research Ctr. (Australia)
Claudia Sharp, WARP Research Ctr. (Australia)
Stephen Moore, WARP Research Ctr. (Australia)
Krishna Mann, Lions Eye Institute (Australia)
Piroska Rakoczy, Lions Eye Institute (Australia)
Ian J. Constable, WARP Researach Ctr. and Lions Eye Institute (Australia)

Published in SPIE Proceedings Vol. 2674:
Laser-Inflicted Eye Injuries: Epidemiology, Prevention, and Treatment
Bruce E. Stuck; Michael Belkin M.D., Editor(s)

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