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Vectorial theory of confocal fluorescence
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Paper Abstract

Imaging in fluorescence microscopy is analyzed using a vectorial diffraction theory. Both conventional and confocal microscopy are considered. A fluorescent molecule is modeled as a radiating electric dipole. Two particular limiting cases are considered: the dipole can either freely rotate in space between excitation and emission, or is fixed in space. For each case, we average over all dipole orientations.

Paper Details

Date Published: 10 April 1996
PDF: 7 pages
Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); doi: 10.1117/12.237488
Show Author Affiliations
Colin J. R. Sheppard, Univ. of Sydney (Australia)
Peter Torok, Univ. of Oxford (United Kingdom)

Published in SPIE Proceedings Vol. 2655:
Three-Dimensional Microscopy: Image Acquisition and Processing III
Carol J. Cogswell; Gordon S. Kino; Tony Wilson, Editor(s)

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