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Proceedings Paper

Time-resolved excimer fluorescence spectroscopy in lipid membranes
Author(s): Kwan Hon Cheng; Lucy Ruymgaart; Digala M. Kulawansa; Pentti Somerharju
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Paper Abstract

Free or conjugated pyrene molecules exhibit concentration dependent excimer fluorescence property. In membrane biophysics, single chain pyrene labeled fluorophores have been extensively used to investigate various physical properties of membranes, e.g., fusion, lateral distribution, stability, polymorphic phase behavior and lipid/protein interactions. Recently, intramolecular excimer forming dual-chain labeled dipyrenyl lipids have been proposed to probe the local conformation and dynamics of membranes. By systematically varying the length of pyrenyl chains of the probes, new information pertaining to the transverse distributions of nanosecond-resolved molecular dynamics of conjugated pyrene molecules can be revealed with spatial resolution approaching 2 - 4 angstrom along the direction perpendicular to the lipid/water interface. In this study, a 3-state kinetic model and a novel nonlinear least squares global analysis method have been used to recover the intralipid free volume and rotational and translational diffusion constants of conjugated pyrenes from the monomer and excimer fluorescence decays and steady state fluorescence intensity data. The transverse distributions of those intramolecular dynamics parameters in membranes with and without local curvatures are presented.

Paper Details

Date Published: 25 March 1996
PDF: 12 pages
Proc. SPIE 2705, Fluorescence Detection IV, (25 March 1996); doi: 10.1117/12.236182
Show Author Affiliations
Kwan Hon Cheng, Texas Tech Univ. (United States)
Lucy Ruymgaart, Texas Tech Univ. (United States)
Digala M. Kulawansa, Texas Tech Univ. (United States)
Pentti Somerharju, Univ. of Helsinki (Finland)

Published in SPIE Proceedings Vol. 2705:
Fluorescence Detection IV
E. Roland Menzel; Abraham Katzir, Editor(s)

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