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Proceedings Paper

Design of environment-responsive biomolecular systems
Author(s): Masuo Aizawa; T. Niimi; T. Haruyama; E. Kobatake
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Paper Abstract

Two different types of biomolecular network systems have been designed to respond to the environmental conditions. One is the calmodulin and enzyme (phosphodiesterase, PDE) that activates phosphodiesterase through the conformational change in responding calcium ion. Calmodulin was genetically engineered to be fused with glutathione-S-transferase (GST). Calmodulin/GST fused protein was self-assembled on the gold surface through glutathione. The calmodulin/GST protein layer exhibited an ability to modulate the PDE activity in a solution phase depending on the calcium ion concentration. The other is the engineered gene structure that produces firefly luciferase in responding environmental pollutants. A TOL plasmid, encoding a binding protein xyl R for xyline and a marker enzyme firefly luciferase, has been implemented in a bacterial cell. The whole cell responded to environmentally hazardous substances such as xylene in emitting light.

Paper Details

Date Published: 9 February 1996
PDF: 7 pages
Proc. SPIE 2716, Smart Structures and Materials 1996: Smart Materials Technologies and Biomimetics, (9 February 1996); doi: 10.1117/12.232133
Show Author Affiliations
Masuo Aizawa, Tokyo Institute of Technology (Japan)
T. Niimi, Tokyo Institute of Technology (Japan)
T. Haruyama, Tokyo Institute of Technology (Japan)
E. Kobatake, Tokyo Institute of Technology (Japan)

Published in SPIE Proceedings Vol. 2716:
Smart Structures and Materials 1996: Smart Materials Technologies and Biomimetics
Andrew Crowson, Editor(s)

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