Precise assessment of wound healing degree is important to avoid infection and achieve good prognosis without remaining of keloid or hypertrophic scars. In this paper, we apply the combination of second-harmonic-generation (SHG) microscopy with third-harmonic-generation (THG) microscopy for incised wound animal model, and visualize collagen dynamics during the wound healing process in time series in situ. Due to the difference of contrast mechanism, SHG image visualizes distribution of dermal collagen fibers whereas THG image visualizes surface of the epidermis. SHG/THG microscopy enable us to track the phase of wound healing for the same subject from the viewpoint of the temporal and spatial dynamic of collagen fibers and the epidermis surface.

Date Published: 24 April 2018
PDF: 4 pages
Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107111E (24 April 2018); doi: 10.1117/12.2319458

Published in SPIE Proceedings Vol. 10711:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo, Editor(s)