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Proceedings Paper

Lateral spatial resolution improvement in laser scanning fluorescence microscopy using a subdiffraction limit optical spot
Author(s): Takahiro Nishimura; Daiki Shinkawa; Yusuke Ogura; Yosuke Tamada; Jun Tanida
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Paper Abstract

This paper presents lateral spatial resolution improvement by scanning a focused spot array pattern. To enhance the effective point spread function (PSF), we employed a phase-only computer generated hologram (CGH) that can reduce the spot size comparing to the single diffraction limited spot. A CGH was designed based on the Gerchberg-Saxton algorithm with a specific constraint to control the dispersion of light energy and the phase of generated spots. As a design example, we obtained a CGH for generating 3x3 optical spots whose sizes were reduced to 78.5% of the single diffraction limited spot. We also confirmed by simulation that the effective PSF was improved from 208 nm to 183 nm when using the subdiffraction limit spots for excitation.

Paper Details

Date Published: 24 April 2018
PDF: 2 pages
Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 1071109 (24 April 2018); doi: 10.1117/12.2319336
Show Author Affiliations
Takahiro Nishimura, Osaka Univ. (Japan)
Daiki Shinkawa, Osaka Univ. (Japan)
Yusuke Ogura, Osaka Univ. (Japan)
Yosuke Tamada, National Institute for Basic Biology (Japan)
Jun Tanida, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 10711:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo, Editor(s)

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