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Proceedings Paper

Observation of immunostained microtubules using three-dimensional super-resolution microscope with two-color annular wave plate
Author(s): Yoshinori Iketaki; Nandor Bokor; Daisuke Okada; Hiroshi Kumagai
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Paper Abstract

Three-dimensional super-resolution microscopy based on fluorescence depletion (3D-SRM) was applied to the observation of immunostained microtubules having complicated structures stacking each other. Owing to a high depth resolution overcoming the diffraction limit, we can observe the tubulin fibers intertwined in a cell with improved image quality. Since the 3D-SRM system can be setup by adopting a simple two-color annular wave plate (TAWP) to a commercial laser scanning microscope (LSM), 3D-SRM is expected to be a powerful measurement method in life science.

Paper Details

Date Published: 24 April 2018
PDF: 5 pages
Proc. SPIE 10712, Optical Manipulation Conference, 1071203 (24 April 2018); doi: 10.1117/12.2319154
Show Author Affiliations
Yoshinori Iketaki, Olympus Corp. (Japan)
Nandor Bokor, Budapest Univ. of Technology and Economics (Hungary)
Daisuke Okada, Kitasato Univ. School of Medicine (Japan)
Hiroshi Kumagai, Kitasato Univ., School of Allied Health Sciences Physics (Japan)

Published in SPIE Proceedings Vol. 10712:
Optical Manipulation Conference
Takashige Omatsu; Hajime Ishihara; Keiji Sasaki, Editor(s)

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