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Proceedings Paper

Isotropic quantitative differential phase contrast microscopic imaging
Author(s): Yu-Zi Lin; Hsi-Hsun Chen; Kuang-Yuh Huang; Yuan Luo
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Paper Abstract

Differential phase contrast microscopic (DPCM) imaging is a popular methodology to recover quantitative phase image of thin transparent sample under multi-axis intensity measurements. To improve the accuracy and stability of phase recovery in conventional DPCM imaging effectively, we propose a new illumination method to achieve isotropic differential phase contrast microscopic (iDPCM) imaging efficiently. Our iDPCM imaging is implemented with a partially coherent microscopy, and a programmable thin-film transistor panel to modulate the illumination pattern. We demonstrate our theoretical approach for iDPCM imaging and experimental results of quantitative phase image of a microlens array and unstained live human cells.

Paper Details

Date Published: 24 April 2018
PDF: 3 pages
Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107110R (24 April 2018); doi: 10.1117/12.2316751
Show Author Affiliations
Yu-Zi Lin, National Taiwan Univ. (Taiwan)
Hsi-Hsun Chen, National Taiwan Univ. (Taiwan)
Kuang-Yuh Huang, National Taiwan Univ. (Taiwan)
Yuan Luo, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 10711:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo, Editor(s)

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