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Proceedings Paper

Multi-focal holographic slit confocal fluorescence microscopy
Author(s): Hung-Chun Wang; Chou-Min Chia; Yu-hsin Chia; Yuan Luo
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Paper Abstract

Confocal microscopy has been widely used to acquire optical sectioning fluorescent image. However, traditional confocal technique requires point-by-point scanning which is time consuming. Alternative techniques to confocal microscopy, such as structured illumination, exist for fast sectioning images, but they require multiple axial planes to be imaged individually. Here, a non-axial line-scanning multifocal confocal microscopy is presented. The proposed system incorporates multiplex volume holographic grating (MVHG) in illumination and combination of multifocal image system. The detailed explanation for resolution on depth axial and simulation results are compared. Also both XY resolution is verified through resolution target. The ability of the proposed system to optical sectioning and multi-depth resolve image of fluorescently labeled microsphere and cornea is experimentally demonstrated.

Paper Details

Date Published: 24 April 2018
PDF: 3 pages
Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 1071108 (24 April 2018); doi: 10.1117/12.2316615
Show Author Affiliations
Hung-Chun Wang, National Tsing Hua Univ. (Taiwan)
Chou-Min Chia, National Taiwan Univ. (Taiwan)
Yu-hsin Chia, National Taiwan Univ. (Taiwan)
Yuan Luo, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 10711:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo, Editor(s)

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