The sensitivity of the Attenuated Total Reflection (ATR) technique to surface modifications allows the study of biological or medical phenomena where solutions seggregate to form a layer on the walls of the sample holder. An aluminum thin-film is deposited at the bottom of the container where the reaction takes place and is attached to a prism. During the reaction a layer is adsorbed on the aluminum film. The study of the total reflectivity in this system when the incoming light enters through the prism shows a resonant absorption peak that shifts towards larger angles of incidence when monochromatic light is used. This shift is related to the optical properties and the thickness of the adsorbed layers and may be quantified. We present here the study of Antigen-Antibody reactions with the aid of this technique.

Date Published: 1 September 1996
PDF: 2 pages
Proc. SPIE 2778, 17th Congress of the International Commission for Optics: Optics for Science and New Technology, 2778E5 (1 September 1996); doi: 10.1117/12.2316198

Published in SPIE Proceedings Vol. 2778:
17th Congress of the International Commission for Optics: Optics for Science and New Technology
Joon-Sung Chang; Jai-Hyung Lee; ChangHee Nam, Editor(s)