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Proceedings Paper

Image enhancement of microscopic fluorescence images
Author(s): Volkmar Betz; Joerg-Uwe Meyer
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Paper Abstract

Two methods are proposed to improve microscopic fluorescence images. In the first method, the second derivative of the image is taken with respect to the focal depth. To obtain the derivative, the focus of the microscope objective is modulated piezoelectrically by 5 - 10 micrometer, and three images with focal positions z plus h, z and z minus h are taken. The resulting image is calculated according to the formula h2 f'(z) approximately equals 2f(z)-f(z plus h)- f(z minus h) that follows immediately from Taylors formula. In the second method, a 2D- Fourier transform is applied to the image, followed by multiplication with a model function that reduces low spatial frequencies. Then, the image is transformed back into the spatial domain. The improvements are demonstrated on fluorescence microscopical images of bronchial tumor tissue.

Paper Details

Date Published: 15 January 1996
PDF: 6 pages
Proc. SPIE 2628, Optical and Imaging Techniques for Biomonitoring, (15 January 1996); doi: 10.1117/12.230021
Show Author Affiliations
Volkmar Betz, Fraunhofer-Institut fuer Biomedizinische Technik (Germany)
Joerg-Uwe Meyer, Fraunhofer-Institut fuer Biomedizinische Technik (Germany)

Published in SPIE Proceedings Vol. 2628:
Optical and Imaging Techniques for Biomonitoring
Hans-Jochen Foth; Renato Marchesini; Halina Podbielska M.D.; Michel Robert-Nicoud; Herbert Schneckenburger, Editor(s)

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