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Proceedings Paper

New capillary optical fiber structure for fluorescence sensors
Author(s): Pauli Kiiveri; Ari Petteri Hokkanen; Raija Kylmanen; Kari Keinanen; Simo K. T. Tammela
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Paper Abstract

A multilayer capillary fiber was designed for optical sensor applications and its optical properties were evaluated by using a fluorescent layer immobilized on its inner surface. This fiber structure combines a large interaction surface (the inner wall of the capillary fiber) for binding of fluorescent indicators with evanescent wave fluorescence measurement, which may facilitate the design of pseudohomogeneous assays without separation of the bound from nonbound fluorescent indicator. The inner surface of the capillary was derivatized by aminosilanization, followed by biotinylation and addition of streptavidin. This biotin- streptavidin coating facilities subsequent immobilization of any biotinylated species (e.g. antibodies, antigens, etc.) participating in specific molecular recognition. We have evaluated some properties of this capillary fiber design by using fluorescent proteins immobilized on the inner wall of capillary by biotin-avidin-interaction. Fluorescence was excited by a HeNe-laser (fluorescent indicator APC; (lambda) em equals 660 nm) and by a Ar-laser (fluorescent indicator RPE, (lambda) em equals 578 nm), and measured with a spectrum analyzer.

Paper Details

Date Published: 10 January 1996
PDF: 11 pages
Proc. SPIE 2695, Functional Photonic and Fiber Devices, (10 January 1996); doi: 10.1117/12.229944
Show Author Affiliations
Pauli Kiiveri, VTT Electronics (Finland)
Ari Petteri Hokkanen, VTT Electronics (Finland)
Raija Kylmanen, VTT Biotechnology and Food Research (Finland)
Kari Keinanen, VTT Biotechnology and Food Research (Finland)
Simo K. T. Tammela, VTT Electronics (Finland)

Published in SPIE Proceedings Vol. 2695:
Functional Photonic and Fiber Devices
S. Iraj Najafi; Mario Nicola Armenise, Editor(s)

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