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Proceedings Paper

High-throughput isotropic mapping of whole mouse brain using multi-view light-sheet microscopy
Author(s): Jun Nie; Yusha Li; Fang Zhao; Junyu Ping; Sa Liu; Tingting Yu; Dan Zhu; Peng Fei
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Paper Abstract

Light-sheet fluorescence microscopy (LSFM) uses an additional laser-sheet to illuminate selective planes of the sample, thereby enabling three-dimensional imaging at high spatial-temporal resolution. These advantages make LSFM a promising tool for high-quality brain visualization. However, even by the use of LSFM, the spatial resolution remains insufficient to resolve the neural structures across a mesoscale whole mouse brain in three dimensions. At the same time, the thick-tissue scattering prevents a clear observation from the deep of brain. Here we use multi-view LSFM strategy to solve this challenge, surpassing the resolution limit of standard light-sheet microscope under a large field-of-view (FOV). As demonstrated by the imaging of optically-cleared mouse brain labelled with thy1-GFP, we achieve a brain-wide, isotropic cellular resolution of ~3μm. Besides the resolution enhancement, multi-view braining imaging can also recover complete signals from deep tissue scattering and attenuation. The identification of long distance neural projections across encephalic regions can be identified and annotated as a result.

Paper Details

Date Published: 12 February 2018
PDF: 6 pages
Proc. SPIE 10481, Neural Imaging and Sensing 2018, 104811X (12 February 2018); doi: 10.1117/12.2295273
Show Author Affiliations
Jun Nie, Huazhong Univ. of Science and Technology (China)
Yusha Li, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Fang Zhao, Huazhong Univ. of Science and Technology (China)
Junyu Ping, Huazhong Univ. of Science and Technology (China)
Sa Liu, Huazhong Univ. of Science and Technology (China)
Tingting Yu, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Dan Zhu, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Peng Fei, Huazhong Univ. of Science and Technology (China)
Britton Chance Ctr. for Biomedical Photonics (China)


Published in SPIE Proceedings Vol. 10481:
Neural Imaging and Sensing 2018
Qingming Luo; Jun Ding, Editor(s)

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