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Proceedings Paper

Approaches from a single- to a multiparameter evanescent wave sensor
Author(s): Daniel Bock; Andreas Hartmann; Sepp Kaul; Michael Martin; V. Schulz; Juergen M. Wolfrum; Stefan Seeger
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Paper Abstract

Evanescent wave sensors are an interesting tool for the fast detection of reactions between biomolecules. By using small and inexpensive diode lasers together with fluorescent dyes in the red spectral region, it was possible to construct a highly specific sensor. This was due to the use of the immobilization of receptor molecules via the Langmuir-Blodgett technique which enhances the specificity drastically. As a clinically relevant system an immunoassay of the tumor marker mucine was investigated. Mucine could be detected with a sandwich test using the antibody system BM-2/BM-7. In order to detect several analytes at one time, there are two possible ways: time-resolved detection using multiplex-dyes and the parallelization of the sensor by using several fibers simultaneously. A position-resolved evanescent wave sensor using a CCD camera is described.

Paper Details

Date Published: 8 January 1996
PDF: 12 pages
Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); doi: 10.1117/12.229506
Show Author Affiliations
Daniel Bock, Ruprecht-Karls-Univ. Heidelberg (Germany)
Andreas Hartmann, Ruprecht-Karls-Univ. Heidelberg (Germany)
Sepp Kaul, Universitaetsfrauenklinik Heidelberg (Germany)
Michael Martin, Universitaetsfrauenklinik Heidelberg (Germany)
V. Schulz, Ruprecht-Karls-Univ. Heidelberg (Germany)
Juergen M. Wolfrum, Ruprecht-Karls-Univ. Heidelberg (Germany)
Stefan Seeger, Ruprecht-Karls-Univ. Heidelberg (Germany)

Published in SPIE Proceedings Vol. 2629:
Biomedical Optoelectronics in Clinical Chemistry and Biotechnology
Stefan Andersson-Engels; Mario Corti; Ivan Kertesz; Norbert Kroo; Heinz P. Weber; Terence A. King; Riccardo Pratesi; Stefan Seeger, Editor(s)

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