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Proceedings Paper

Optimization of fluorimetric lipid membrane biosensor sensitivity through manipulation of membrane structure and nitrobenzoxadiazole dipalmitoylphosphatidylethanolamine concentration
Author(s): Jason D. A. Shrive; Ulrich J. Krull
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Paper Abstract

In the work reported here, surface concentrations of 0.027 and 0.073 molecules nm-2 of the fluorescent membrane probe molecule nitrobenzoxadiazole dipalmitoylphosphatidylethanolamine (NBD-PE) were shown to yield optimum sensitivity for fluorimetric transduction of membrane structural perturbations for lipid membrane-based biosensor development. These optima were obtained through correlation of experimental data with theoretical predictions of optimum surface concentrations based on a model for NBD-PE self quenching previously published by our group. It was also determined that membrane structural heterogeneity improves the sensitivity of NBD-PE labeled membrane transducers. Together with fluorescence microscopy, observations of surface potential change upon compression or expansion of phosphatidylcholine (PC)/phosphatidic acid (PA) monolayers were used to qualitatively indicate the degree of structural heterogeneity in these membranes. It was determined that sub-microscopic domains must exist in microscopically homogeneous egg PC/egg PA membranes in order to facilitate the observed NBD-PE self-quenching responses upon alteration of bulk pH and therefore, membrane surface electrostatics and structure.

Paper Details

Date Published: 3 January 1995
PDF: 12 pages
Proc. SPIE 2631, Medical and Fiber Optic Sensors and Delivery Systems, (3 January 1995); doi: 10.1117/12.229189
Show Author Affiliations
Jason D. A. Shrive, Univ. of Toronto (Canada)
Ulrich J. Krull, Univ. of Toronto (Canada)

Published in SPIE Proceedings Vol. 2631:
Medical and Fiber Optic Sensors and Delivery Systems
Nathan I. Croitoru; Mitsunobu Miyagi; Guillermo Orellana; Annamaria Verga Scheggi; Henricus J. C. M. Sterenborg, Editor(s)

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