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Proceedings Paper

An all-optical system for rapid and deep interrogation of behaviorally relevant activity patterns (Conference Presentation)
Author(s): Gilad Lerman; Jonathan V. Gill; Dmitry Rinberg; Shy Shoham

Paper Abstract

Holographic optogenetics is an emerging tool for distributed control of spatiotemporal neuronal activity. Establishing causality between specific sequences of neuronal activity and behavior requires manipulating this code at the level of individual neurons while recording neural responses and behavioral readout. However, traditional methods of optogenetic perturbation lack the ability to emulate natural patterns of neural activity, or to rapidly alter the activity of specific neurons deep in the brain. To address the goal of producing behaviorally relevant sequences of neural activity, we have developed an all-optical, rapid two-photon optogenetic stimulation and imaging system with cellular resolution and 5 ms temporal precision. Using an amplified laser with high peak pulse power, together with wavefront shaping methods using a fast spatial light modulator (3 ms switching time), we were able to stimulate dozens of neurons deep in the olfactory bulb (>350 µm) at a high rate (>100 Hz) with cellular resolution. We optimized the system parameters to enable efficient excitation with a low power budget, to enable the simultaneous stimulation of many cells (~60). We then demonstrated stimulation of mitral and tufted cells, the projection neurons of the olfactory bulb, at a high rate, generating artificial odor-evoked responses. We will present the system characteristics and discuss its potential applications for manipulating and reading neuronal activity on a behaviorally relevant spatiotemporal scale to dissect the activity codes that guide behavior across different modalities.

Paper Details

Date Published: 14 March 2018
Proc. SPIE 10482, Optogenetics and Optical Manipulation 2018, 104820U (14 March 2018); doi: 10.1117/12.2290850
Show Author Affiliations
Gilad Lerman, New York Univ. School of Medicine (United States)
Jonathan V. Gill, New York Univ. School of Medicine (United States)
Dmitry Rinberg, New York Univ. School of Medicine (United States)
Shy Shoham, Technion-Israel Institute of Technology (Israel)

Published in SPIE Proceedings Vol. 10482:
Optogenetics and Optical Manipulation 2018
Samarendra K. Mohanty; Nitish V. Thakor; E. Duco Jansen, Editor(s)

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