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Proceedings Paper

Multimodality optical coherence tomography and fluorescence confocal scanning laser ophthalmoscopy for image-guided feedback of intraocular injections in mouse models
Author(s): Oscar R. Benavides; Benjamin D. Terrones; Kelsey C. Leeburg; Sankarathi B. Mehanathan; Edward M. Levine; Yuankai K. Tao
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Paper Abstract

Rodent models are robust tools for understanding human retinal disease and function because of their similarities with human physiology and anatomy and availability of genetic mutants. Optical coherence tomography (OCT) has been well-established for ophthalmic imaging in rodents and enables depth-resolved visualization of structures and image-based surrogate biomarkers of disease. Similarly, fluorescence confocal scanning laser ophthalmoscopy (cSLO) has demonstrated utility for imaging endogenous and exogenous fluorescence and scattering contrast in the mouse retina. Complementary volumetric scattering and en face fluorescence contrast from OCT and cSLO, respectively, enables cellular-resolution longitudinal imaging of changes in ophthalmic structure and function. We present a non-contact multimodal OCT+cSLO small animal imaging system with extended working distance to the pupil, which enables imaging during and after intraocular injection. While injections are routinely performed in mice to develop novel models of ophthalmic diseases and screen novel therapeutics, the location and volume delivered is not precisely controlled and difficult to reproduce. Animals were imaged using a custom-built OCT engine and scan-head combined with a modified commercial cSLO scan-head. Post-injection imaging showed structural changes associated with retinal puncture, including the injection track, a retinal elevation, and detachment of the posterior hyaloid. When combined with imagesegmentation, we believe OCT can be used to precisely identify injection locations and quantify injection volumes. Fluorescence cSLO can provide complementary contrast for either fluorescently labeled compounds or transgenic cells for improved specificity. Our non-contact OCT+cSLO system is uniquely-suited for concurrent imaging with intraocular injections, which may be used for real-time image-guided injections.

Paper Details

Date Published: 19 February 2018
PDF: 4 pages
Proc. SPIE 10474, Ophthalmic Technologies XXVIII, 104741G (19 February 2018); doi: 10.1117/12.2290663
Show Author Affiliations
Oscar R. Benavides, Vanderbilt Univ. (United States)
Benjamin D. Terrones, Vanderbilt Univ. (United States)
Kelsey C. Leeburg, Vanderbilt Univ. (United States)
Sankarathi B. Mehanathan, Vanderbilt Univ. (United States)
Edward M. Levine, Vanderbilt Univ. (United States)
Yuankai K. Tao, Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 10474:
Ophthalmic Technologies XXVIII
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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