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Proceedings Paper

Segmentation and analysis of mouse pituitary cells with graphic user interface (GUI)
Author(s): Erika González; Lucía Medina; Mathieu Hautefeuille; Tatiana Fiordelisio
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Paper Abstract

In this work we present a method to perform pituitary cell segmentation in image stacks acquired by fluorescence microscopy from pituitary slice preparations. Although there exist many procedures developed to achieve cell segmentation tasks, they are generally based on the edge detection and require high resolution images. However in the biological preparations that we worked on, the cells are not well defined as experts identify their intracellular calcium activity due to fluorescence intensity changes in different regions over time. This intensity changes were associated with time series over regions, and because they present a particular behavior they were used into a classification procedure in order to perform cell segmentation.

Two logistic regression classifiers were implemented for the time series classification task using as features the area under the curve and skewness in the first classifier and skewness and kurtosis in the second classifier. Once we have found both decision boundaries in two different feature spaces by training using 120 time series, the decision boundaries were tested over 12 image stacks through a python graphical user interface (GUI), generating binary images where white pixels correspond to cells and the black ones to background. Results show that area-skewness classifier reduces the time an expert dedicates in locating cells by up to 75% in some stacks versus a 92% for the kurtosis-skewness classifier, this evaluated on the number of regions the method found. Due to the promising results, we expect that this method will be improved adding more relevant features to the classifier.

Paper Details

Date Published: 20 February 2018
PDF: 13 pages
Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104971T (20 February 2018); doi: 10.1117/12.2290298
Show Author Affiliations
Erika González, Univ. Nacional Autónoma de México (Mexico)
Lucía Medina, Univ. Nacional Autónoma de México (Mexico)
Mathieu Hautefeuille, Univ. Nacional Autónoma de México (Mexico)
Tatiana Fiordelisio, Univ. Nacional Autónoma de México (Mexico)

Published in SPIE Proceedings Vol. 10497:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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