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Proceedings Paper

High-speed particle tracking in microscopy using SPAD image sensors
Author(s): Istvan Gyongy; Amy Davies; Allende Miguelez Crespo; Andrew Green; Neale A. W. Dutton; Rory R. Duncan; Colin Rickman; Robert K. Henderson; Paul A. Dalgarno
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Paper Abstract

Single photon avalanche diodes (SPADs) are used in a wide range of applications, from fluorescence lifetime imaging microscopy (FLIM) to time-of-flight (ToF) 3D imaging. SPAD arrays are becoming increasingly established, combining the unique properties of SPADs with widefield camera configurations. Traditionally, the photosensitive area (fill factor) of SPAD arrays has been limited by the in-pixel digital electronics. However, recent designs have demonstrated that by replacing the complex digital pixel logic with simple binary pixels and external frame summation, the fill factor can be increased considerably. A significant advantage of such binary SPAD arrays is the high frame rates offered by the sensors (>100kFPS), which opens up new possibilities for capturing ultra-fast temporal dynamics in, for example, life science cellular imaging. In this work we consider the use of novel binary SPAD arrays in high-speed particle tracking in microscopy. We demonstrate the tracking of fluorescent microspheres undergoing Brownian motion, and in intra-cellular vesicle dynamics, at high frame rates. We thereby show how binary SPAD arrays can offer an important advance in live cell imaging in such fields as intercellular communication, cell trafficking and cell signaling.

Paper Details

Date Published: 20 February 2018
PDF: 6 pages
Proc. SPIE 10505, High-Speed Biomedical Imaging and Spectroscopy III: Toward Big Data Instrumentation and Management, 105050A (20 February 2018); doi: 10.1117/12.2290199
Show Author Affiliations
Istvan Gyongy, The Univ. of Edinburgh (United Kingdom)
Amy Davies, Heriot-Watt Univ. (United Kingdom)
Allende Miguelez Crespo, Heriot-Watt Univ. (United Kingdom)
Andrew Green, Heriot-Watt Univ. (United Kingdom)
Neale A. W. Dutton, STMicroelectronics (R&D) Ltd. (United Kingdom)
Rory R. Duncan, Heriot-Watt Univ. (United Kingdom)
Colin Rickman, Heriot-Watt Univ. (United Kingdom)
Robert K. Henderson, The Univ. of Edinburgh (United Kingdom)
Paul A. Dalgarno, Heriot-Watt Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 10505:
High-Speed Biomedical Imaging and Spectroscopy III: Toward Big Data Instrumentation and Management
Kevin K. Tsia; Keisuke Goda, Editor(s)

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