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Proceedings Paper

Fluorescence lifetime imaging of cells with bound and internalized fluorescent gold nanoclusters (Conference Presentation)
Author(s): Marina Mutas; Christian Strelow; Tobias Kipp; Alf Mews

Paper Abstract

Fluorescent gold nanoclusters (AuNCs) are novel promising nanomaterials for biomedical applications because of their unique physiochemical properties. We use AuNCs stabilized with mercaptoundecanoic acid (MUA-AuNCs) which show a fluorescence emission that peaks at a wavelength around 525 nm, which is in the same wavelength region as the autofluorescence of a biological cell. However, the fluorescence decay time of the MUA-AuNCs (100 ns) is much longer in comparison to the autofluorescence (3 ns). Fluorescence lifetime imaging microscopy (FLIM) is a powerful method to discriminate between emitters of different fluorescence lifetimes. We biofunctionalize these MUA-AuNCs with biomolecules that specifically bind to the receptor expressed on the cell's membrane. To get an image of the whole cell and the bound and internalized AuNCs we use cross-sectional FLIM scans in axial direction at different heights through the cell. We distinguish between specifically bound and internalized MUA-AuNCs on and in cells by means of different FLIM methods supported by element-specific scanning electron microscopy and semi-empirical simulations.

Paper Details

Date Published: 15 March 2018
Proc. SPIE 10507, Colloidal Nanoparticles for Biomedical Applications XIII, 1050717 (15 March 2018); doi: 10.1117/12.2290067
Show Author Affiliations
Marina Mutas, Univ. Hamburg (Germany)
Christian Strelow, Univ. Hamburg (Germany)
Tobias Kipp, Univ. Hamburg (Germany)
Alf Mews, Univ. Hamburg (Germany)

Published in SPIE Proceedings Vol. 10507:
Colloidal Nanoparticles for Biomedical Applications XIII
Marek Osiński; Wolfgang J. Parak; Xing-Jie Liang, Editor(s)

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