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Proceedings Paper

Frequency-domain photoacoustic and fluorescence microscopy: application on labeled and unlabeled cells
Author(s): Gregor Langer; Bianca Buchegger; Jaroslaw Jacak; Karoline Pfeffer; Sven Wohlfarth; Günther Hannesschläger; Thomas A. Klar; Thomas Berer
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Paper Abstract

In this paper, multimodal optical-resolution frequency-domain photoacoustic and fluorescence scanning microscopy is presented on labeled and unlabeled cells. In many molecules, excited electrons relax radiatively and non-radiatively, leading to fluorescence and photoacoustic signals, respectively. Both signals can then be detected simultaneously. There also exist molecules, e.g. hemoglobin, which do not exhibit fluorescence, but provide photoacoustic signals solely. Other molecules, especially fluorescent dyes, preferentially exhibit fluorescence. The fluorescence quantum yield of a molecule and with it the strength of photoacoustic and fluorescence signals depends on the local environment, e.g. on the pH. Therefore, the local distribution of the simultaneously recorded photoacoustic and fluorescence signals may be used in order to obtain information about the local chemistry.

Paper Details

Date Published: 13 February 2018
PDF: 7 pages
Proc. SPIE 10487, Multimodal Biomedical Imaging XIII, 1048709 (13 February 2018); doi: 10.1117/12.2290023
Show Author Affiliations
Gregor Langer, Research Ctr. for Non Destructive Testing GmbH (Austria)
Bianca Buchegger, Johannes Kepler Univ. Linz (Austria)
Jaroslaw Jacak, Johannes Kepler Univ. Linz (Austria)
Univ. of Applied Sciences Upper Austria (Austria)
Karoline Pfeffer, Tierärztliche Praxis Claudia und Michael Grötzschel (Austria)
Sven Wohlfarth, Research Ctr. for Non Destructive Testing GmbH (Austria)
Günther Hannesschläger, Research Ctr. for Non Destructive Testing GmbH (Austria)
Thomas A. Klar, Johannes Kepler Univ. Linz (Austria)
Thomas Berer, Research Ctr. for Non Destructive Testing GmbH (Austria)

Published in SPIE Proceedings Vol. 10487:
Multimodal Biomedical Imaging XIII
Fred S. Azar; Xavier Intes, Editor(s)

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