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Proceedings Paper

Control of intracellular ionic concentrations by mid-infrared laser irradiation
Author(s): G. Takebe; T. Yamauchi; Y. Shimizu; T. Dougakiuchi
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Paper Abstract

We successfully induced intracellular ion concentration changes in live culture cells using mid-infrared laser irradiation. The laser used for irradiation was a quantum cascade laser with a wavelength of 6.1 micrometers. We tuned the power of the laser to be between 30 to 60 mW at the sample. Cell lines, namely HeLa and Chinese hamster ovary cell lines, were used. They were cultured on specially fabricated silicon-bottom dishes. Live cells were stained using ion-sensitive dyes such as Calcium Green-1. The mid-infrared light was incident on the cell samples from the bottom of the dish through the silicon plate, and fluorescence imaging of the ion concentrations was performed using an upright fluorescence microscope placed on top of the sample stage. The mid-infrared lasers were operated in the continuous wave mode and light irradiations onto the cells were temporally controlled using a mechanical shutter in a periodical on-and-off pattern in the second timescale. The cells showed oscillations in their ionic concentration, which was synchronized with the periodical mid-infrared irradiation, and the threshold power needed for evoking the ion concentration change was dependent on the cell types and ion species. These results demonstrated that mid-infrared light directly changed the ionic response within cells and had the ability to change cell functions.

Paper Details

Date Published: 13 February 2018
PDF: 4 pages
Proc. SPIE 10492, Optical Interactions with Tissue and Cells XXIX, 104920G (13 February 2018);
Show Author Affiliations
G. Takebe, Hamamatsu Photonics K.K. (Japan)
T. Yamauchi, Hamamatsu Photonics K.K. (Japan)
Y. Shimizu, Hamamatsu Photonics K.K. (Japan)
T. Dougakiuchi, Hamamatsu Photonics K.K. (Japan)

Published in SPIE Proceedings Vol. 10492:
Optical Interactions with Tissue and Cells XXIX
E. Duco Jansen; Hope Thomas Beier, Editor(s)

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