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Proceedings Paper

Photodynamic diagnostics of stress-induced gastrointestinal neoplasia in laboratory animals using 5-aminolevulinic acid and Al-phthalocyanine
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Paper Abstract

The main research objective is the development of innovative optical technologies for sensitive diagnosis of early stages of development of stomach cancer and monitoring of stress-induced appearance and development of tumors of the gastrointestinal tract by applying endogenous and exogenous fluorescence spectroscopy modalities.

Different mechanisms solely and in combination for evaluation of the joint impact of bioenvironmental factors (stress, Helicobacter pillory, exo-toxins in the food, water, soil and air) were applied to induce gastrointestinal tract (GIT) neoplasia in rats. The transformation of damaged areas of the stomach mucosa into malignancies in all parts of gastrointestinal tract were detected using exogenous fluorescence of photosensitizers - 5-aminolevulinic acid (5-ALA) and aluminum phthalocyanine (Al-Pc). Fluorescent mapping of different organs (liver, spleen, lungs, brain) also was developed – to evaluate the distribution of the photosensitizers in the whole body on the second hour after photosensitizer application by intravenous injection. Fiber-optic probe was used to measure the organs investigated. Fluorescence spectra were detected by microspectrometer USB4000 (OceanOptics Inc., USA), and FS405 LED source on 405 nm was used as excitation source for both types of photosensitizers applied.

Diagnostically-important parameters of oximetry, optical coherence tomography and speckle-imaging of the microcirculation of the stomach were also evaluated, to evaluate changes in the blood flow and vascular architecture, during the formation of the initial phases of the neoplasm development.

Paper Details

Date Published: 20 February 2018
PDF: 6 pages
Proc. SPIE 10501, Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics, 105011E (20 February 2018); doi: 10.1117/12.2289159
Show Author Affiliations
Ekaterina Borisova, Institute of Electronics (Bulgaria)
Saratov National Research State Univ. (Russian Federation)
Oxana Semyachkina-Glushkovskaya, Saratov National Research State Univ. (Russian Federation)
Nikita Navolokin, Saratov State Medical Univ. (Russian Federation)
Vanya Mantareva, Institute of Organic Chemistry with the Ctr. of Phytochemistry (Bulgaria)
Ivan Angelov, Institute of Organic Chemistry with the Ctr. of Phytochemistry (Bulgaria)
Ilana Agranovich, Saratov National Research State Univ. (Russian Federation)
Alexander Khorovodov, Saratov National Research State Univ. (Russian Federation)
Natalia Shushunova, Saratov National Research State Univ. (Russian Federation)
Anastasiya Bodrova, Saratov National Research State Univ. (Russian Federation)
Ivan Fedosov, Saratov National Research State Univ. (Russian Federation)
Anton Namykin, Saratov National Research State Univ. (Russian Federation)
Arkady Abdurashitov, Saratov National Research State Univ. (Russian Federation)
Latchezar Avramov, Institute of Electronics (Bulgaria)


Published in SPIE Proceedings Vol. 10501:
Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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