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Proceedings Paper

Multispectral detection of cutaneous lesions using spectroscopy and microscopy approaches
Author(s): E. Borisova; Ts. Genova-Hristova; P. Troyanova; E. Pavlova; I. Terziev; O. Semyachkina-Glushkovskaya; M. Lomova; E. Genina; G. Stanciu; D. Tranca; L. Avramov
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Paper Abstract

Autofluorescence, diffuse-reflectance and transmission spectral, and microscopic measurements were made on different cutaneous neoplastic lesions, namely basal cell carcinoma, squamous cell carcinoma, malignant melanoma, and dysplastic and benign lesions related. Spectroscopic measurements were made on ex vivo tissue samples, and confocal microscopy investigations were made on thin tissue slices.

Fluorescence spectra obtained reveal statistically significant differences between the different benign, dysplastic and malignant lesions by the level of emission intensity, as well by spectral shape, which are fingerprints applicable for differentiation algorithms. In reflectance mode the most significant differences are related to the influence of skin pigments – melanin and hemoglobin. Transmission spectroscopy mode gave complementary optical properties information about the tissue samples investigated to that one of reflectance and absorption spectroscopy.

Using autofluorescence detection of skin lesions we obtain very good diagnostic performance for distinguishing of nonmelanoma lesions. Using diffuse reflectance and transmission spectroscopy we obtain significant tool for pigmented pathologies differentiation, but it is a tool with moderate sensitivity for non-melanoma lesions detection. One could rapidly increase the diagnostic accuracy of the received combined “optical biopsy” method when several spectral detection techniques are applied in common algorithm for lesions’ differentiation.

Specific spectral features observed in each type of lesion investigated on micro and macro level would be presented and discussed. Correlation between the spectral data received and the microscopic features observed would be discussed in the report.

Paper Details

Date Published: 14 February 2018
PDF: 6 pages
Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018, 104670M (14 February 2018);
Show Author Affiliations
E. Borisova, Institute of Electronics (Bulgaria)
Saratov National Research State Univ. (Russian Federation)
Ts. Genova-Hristova, Institute of Electronics (Bulgaria)
P. Troyanova, Univ. Hospital “Tsaritsa Yoanna – ISUL” (Bulgaria)
E. Pavlova, Univ. Hospital “Tsaritsa Yoanna – ISUL” (Bulgaria)
I. Terziev, Univ. Hospital “Tsaritsa Yoanna – ISUL” (Bulgaria)
O. Semyachkina-Glushkovskaya, Saratov National Research State Univ. (Russian Federation)
M. Lomova, Saratov National Research State Univ. (Russian Federation)
E. Genina, Saratov National Research State Univ. (Russian Federation)
G. Stanciu, Univ. Politehnica of Bucharest (Romania)
D. Tranca, Univ. Politehnica of Bucharest (Romania)
L. Avramov, Institute of Electronics (Bulgaria)

Published in SPIE Proceedings Vol. 10467:
Photonics in Dermatology and Plastic Surgery 2018
Bernard Choi; Haishan Zeng, Editor(s)

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