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Proceedings Paper

Fluorescence lifetime imaging and its applications in cellular microenvironment measurement and auxiliary diagnosis
Author(s): Teng Luo; Svitlana M. Levchenko; Artem Pliss; Xiao Peng; Wei Yan; Paras N. Prasad; Liwei Liu; Junle Qu
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Paper Abstract

We present our recent work on the applications of fluorescence lifetime imaging microscopy(FLIM), including the monitoring of macromolecule dynamic changes in the nucleolar compartments and the auxiliary diagnosis of H and E-stained sections. We demonstrated the capability of FLIM to measure protein concentration in the specific cellular compartments in live cells. We proposed to use FLIM to monitor changes in intracellular protein concentration caused by various factors e.g. cell cycle progression, drug treatment etc. In the future, FLIM technology is expected to be combined with super-resolution optical imaging. FLIM with molecular resolution will have the potential to serve as a powerful tool for discovering new phenomena and revealing new mechanisms in biomedical research, which will effectively promote the development of life science.

Paper Details

Date Published: 19 February 2018
PDF: 9 pages
Proc. SPIE 10495, Biophotonics and Immune Responses XIII, 104950I (19 February 2018);
Show Author Affiliations
Teng Luo, Shenzhen Univ. (China)
Svitlana M. Levchenko, Shenzhen Univ. (China)
Artem Pliss, Univ. at Buffalo (United States)
Xiao Peng, Shenzhen Univ. (China)
Wei Yan, Shenzhen Univ. (China)
Paras N. Prasad, Univ. at Buffalo (United States)
Liwei Liu, Shenzhen Univ. (China)
Junle Qu, Shenzhen Univ. (China)

Published in SPIE Proceedings Vol. 10495:
Biophotonics and Immune Responses XIII
Wei R. Chen, Editor(s)

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