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Proceedings Paper

Quantitative phase imaging characterization of tumor-associated blood vessel formation on a chip
Author(s): Peng Guo; Jing Huang; Marsha A. Moses
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Paper Abstract

Angiogenesis, the formation of new blood vessels from existing ones, is a biological process that has an essential role in solid tumor growth, development, and progression. Recent advances in Lab-on-a-Chip technology has created an opportunity for scientists to observe endothelial cell (EC) behaviors during the dynamic process of angiogenesis using a simple and economical in vitro platform that recapitulates in vivo blood vessel formation. Here, we use quantitative phase imaging (QPI) microscopy to continuously and non-invasively characterize the dynamic process of tumor cell-induced angiogenic sprout formation on a microfluidic chip. The live tumor cell-induced angiogenic sprouts are generated by multicellular endothelial sprouting into 3 dimensional (3D) Matrigel using human umbilical vein endothelial cells (HUVECs). By using QPI, we quantitatively measure a panel of cellular morphological and behavioral parameters of each individual EC participating in this sprouting. In this proof-of-principle study, we demonstrate that QPI is a powerful tool that can provide real-time quantitative analysis of biological processes in in vitro 3D biomimetic devices, which, in turn, can improve our understanding of the biology underlying functional tissue engineering.

Paper Details

Date Published: 23 February 2018
PDF: 9 pages
Proc. SPIE 10503, Quantitative Phase Imaging IV, 105031O (23 February 2018);
Show Author Affiliations
Peng Guo, Boston Children's Hospital (United States)
Harvard Medical School (United States)
Jing Huang, Boston Children's Hospital (United States)
Harvard Medical School (United States)
Marsha A. Moses, Boston Children's Hospital (United States)
Harvard Medical School (United States)

Published in SPIE Proceedings Vol. 10503:
Quantitative Phase Imaging IV
Gabriel Popescu; YongKeun Park, Editor(s)

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