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Proceedings Paper

Optimized fluorescence diagnosis of tumors by comparing 5-ALA-induced xenofluorescence and autofluorescence intensities of a murine tumor/nontumor tissue system cultivated on the CAM
Author(s): Simone Stroebele; Cathrin Dressler; M. Samy Ismail; Anita Daskalaki; Carsten M. Philipp; Hans-Peter Berlien; H. Weitzel; M. Liebsch; H. Spielmann
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Paper Abstract

The in vivo model of the chorioallantoic membrane of fertilized chicken embryos (CAM) was employed for studying the fluorescence characteristics of tumor tissue in comparison with non tumorous tissue. Tumors were grown from the murine fibrosarcoma cell line SSK II and murine 3T3 fibroblasts (clone A31) were used for cultivating non tumorous tissue. Autofluorescence and xenofluorescence intensities induced by 5-aminolaevulinic acid (5-ALA) were compared. Exogenous administration of 5-ALA, an early precursor in haem synthesis, induces accumulation of endogenous photoactive porphyrins, in particular protoporphyrin IX (PpIX). Fluorescence investigations were performed after 3-4d of incubation, when the tissues had reached macroscopically three dimensional stages of growth. Fluorescences were excited with a HBO-X 100 W lamp (Carl Zeiss) at a wavelength (lambda) equals 405 plus or minus 5 nm. Emissions were detected in the spectral range above 630 nm and visualized by real time digital image processing (Argus 10, HAMAMATSU) using an ICCD camera (HAMAMATSU). After administration of 0.4 mmolar 5-ALA solution to the CAM inoculated tissues the SSK II tumors exhibited higher fluorescence intensities than the 3T3 non tumorous tissues. Autofluorescence intensities of both types of tissues were not distinguishable. Furthermore, the effects of several biochemicals on the xenofluorescence intensities of the fibrosarcoma and fibroblast tissues were investigated.

Paper Details

Date Published: 27 December 1995
PDF: 12 pages
Proc. SPIE 2627, Optical Biopsies, (27 December 1995); doi: 10.1117/12.228898
Show Author Affiliations
Simone Stroebele, Freie Univ. Berlin (Germany)
Cathrin Dressler, Freie Univ. Berlin and Laser-Medizin-Technologiezentrum GmbH (Germany)
M. Samy Ismail, Freie Univ. Berlin (Germany)
Anita Daskalaki, Freie Univ. Berlin (Germany)
Carsten M. Philipp, Freie Univ. Berlin (Germany)
Hans-Peter Berlien, Freie Univ. Berlin and Laser-Medizin-Technologiezentrum GmbH (Germany)
H. Weitzel, Freie Univ. Berlin (Germany)
M. Liebsch, Bundesinsitut fuer gesundheitlichen (Germany)
H. Spielmann, Bundesinsitut fuer gesundheitlichen (Germany)

Published in SPIE Proceedings Vol. 2627:
Optical Biopsies
Rinaldo Cubeddu; Serge R. Mordon; Katarina Svanberg M.D., Editor(s)

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