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Proceedings Paper

In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography
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Paper Abstract

Despite obvious improvements in visualization of the in vivo cornea through the faster imaging speeds and higher axial resolutions, cellular imaging stays unresolvable task for OCT, as en face viewing with a high lateral resolution is required. The latter is possible with FFOCT, a method that relies on a camera, moderate numerical aperture (NA) objectives and an incoherent light source to provide en face images with a micrometer-level resolution. Recently, we for the first time demonstrated the ability of FFOCT to capture images from the in vivo human cornea1. In the current paper we present an extensive study of appearance of healthy in vivo human corneas under FFOCT examination. En face corneal images with a micrometer-level resolution were obtained from the three healthy subjects. For each subject it was possible to acquire images through the entire corneal depth and visualize the epithelium structures, Bowman’s layer, sub-basal nerve plexus (SNP) fibers, anterior, middle and posterior stroma, endothelial cells with nuclei. Dimensions and densities of the structures visible with FFOCT, are in agreement with those seen by other cornea imaging methods. Cellular-level details in the images obtained together with the relatively large field-of-view (FOV) and contactless way of imaging make this device a promising candidate for becoming a new tool in ophthalmological diagnostics.

Paper Details

Date Published: 19 February 2018
PDF: 7 pages
Proc. SPIE 10474, Ophthalmic Technologies XXVIII, 104740S (19 February 2018); doi: 10.1117/12.2288947
Show Author Affiliations
Viacheslav Mazlin, Institut Langevin, ESPCI Paris, PSL Research Univ. (France)
Peng Xiao, Institut Langevin, ESPCI Paris, PSL Research Univ. (France)
Eugénie Dalimier, LLTech SAS (France)
Kate Grieve, UPMC-Sorbonne Univ. (France)
Quinze-Vingts National Eye Hospital (France)
Kristina Irsch, UPMC-Sorbonne Univ. (France)
Quinze-Vingts National Eye Hospital (France)
The Wilmer Eye Institute, The Johns Hopkins Univ. School of Medicine (United States)
José Sahel M.D., UPMC-Sorbonne Univ. (United States)
Quinze-Vingts National Eye Hospital (France)
Univ. of Pittsburgh School of Medicine (United States)
Mathias Fink, Institute Langevin, ESPCI Paris, PSL Research Univ. (France)
Claude Boccara, Institute Langevin, ESPCI Paris, PSL Research Univ. (France)
LLTech SAS (France)

Published in SPIE Proceedings Vol. 10474:
Ophthalmic Technologies XXVIII
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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