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Proceedings Paper

Exploring plasmonic coupling as a stimuli responsive contrast mechanism in multiphoton microscopy
Author(s): Marica B. Ericson; Hanna Thomsen; Jeemol James; Vladimir Kirejev; Daniel Aili; Antonio Vargas-Berenguel
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Paper Abstract

A novel approach for optical biosensing can be obtained based multiphoton induced luminescence (MIL) and its dependence on plasmonic coupling. It has been shown that the proximity of spherical AuNPs determines the generation of MIL in far-field multiphoton laser scanning microscopy (MPM). A stimuli responsive contrast mediator with high sensitivity can be created by controlling the aggregated state of AuNP. In this study we explore a system based on spherical AuNPs functionalized with β-cyclodextrin and multiple β-D-lactose units (lacto-CD-AuNP). The aim of the β- D-lactose units is to target cancer cells, based on overexpression of galectin3 (Gal-3) receptors. The results demonstrate that clustering of particles, and thereby MIL signal, was only acquired from tumor cell lines, i.e., SK-MEL-28 and A431, while not from normal keratinocytes (HEKn). Thus further studies should be undertaken to translate the concept to a preclinical setting.

Paper Details

Date Published: 23 February 2018
PDF: 6 pages
Proc. SPIE 10509, Plasmonics in Biology and Medicine XV, 1050907 (23 February 2018);
Show Author Affiliations
Marica B. Ericson, Univ. of Gothenburg (Sweden)
Hanna Thomsen, Univ. of Gothenburg (Sweden)
Jeemol James, Univ. of Gothenburg (Sweden)
Vladimir Kirejev, Univ. of Gothenburg (Sweden)
Fluicell AB (Sweden)
Daniel Aili, Linköping Univ. (Sweden)
Antonio Vargas-Berenguel, Univ. of Almeria (Spain)

Published in SPIE Proceedings Vol. 10509:
Plasmonics in Biology and Medicine XV
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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