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Proceedings Paper

Resonant nanopillars as label-free optical biosensors
Author(s): Ana López-Hernandez; Rafael Casquel; Miguel Holgado ; Iñaki Cornago; Fátima Fernández; Paula Ciaurriz; Francisco J. Sanza; Beatriz Santamaría; Maria V. Maigler; María Fe Laguna
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Paper Abstract

In recent works it has been demonstrated the suitability of using resonant nanopillars (R-NPs) as biochemical. In this work it has been shown the capability of the R-NPs to behave as label-free multiplexed biological sensors. Each R-NP is formed by silicon oxide (SiO2) and silicon nitride (Si3N4) Bragg reflectors and a central cavity of SiO2, and they are grouped into eight arrays called BICELLs, which are distributed on a single chip of quartz substrate for multiplexing measurements. For the biological sensing assessment it was developed an immunoassay on the eight single BICELLs. The biofunctionalization process was performed by a silanization protocol based on 3-aminopropyltrymethoxysilane (APTMS) and glutaradheyde (GA) as a linker between APTMS and the IgG which acted as biorreceptor for the anti-IgG recognition. In this work, there were compared two forms of immobilization: on one hand by incubating the R-NPs under static drop of 50 μg/mL and on the second hand by introducing the sensing chip in a flow cell with a continuous flow of the same concentration of IgG. The eight arrays of R-NPs or BICELLs were independently optically interrogated by a bundle of fiber connected to a spectrometer. The multiplexing analysis showed reproducibility among the BICELLs, suggesting the potentially of using R-NPs for multiplexed biosensors. Performance in the immobilization process apparently does not have a signification effect. However the election of one method or another should be a commitment between time and resources.

Paper Details

Date Published: 14 February 2018
PDF: 7 pages
Proc. SPIE 10488, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVIII, 1048814 (14 February 2018);
Show Author Affiliations
Ana López-Hernandez, Univ. Politécnica de Madrid (Spain)
Rafael Casquel, Univ. Politécnica de Madrid (Spain)
Miguel Holgado , Univ. Politécnica de Madrid (Spain)
Iñaki Cornago, CEMITEC (Spain)
Fátima Fernández, CEMITEC (Spain)
Paula Ciaurriz, CEMITEC (Spain)
Francisco J. Sanza, Univ. Politécnica de Madrid (Spain)
Beatriz Santamaría, Univ. Politécnica de Madrid (Spain)
Maria V. Maigler, Univ. Politécnica de Madrid (Spain)
María Fe Laguna, Univ. Politécnica de Madrid (Spain)

Published in SPIE Proceedings Vol. 10488:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVIII
Israel Gannot, Editor(s)

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