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Proceedings Paper

Phase from defocus
Author(s): Ondrej Mandula; Cédric Allier; Lionel Hervé; Eric Denarier; Anne Fourest-Lieuvin; Sylvie Gory-Fauré; Angélique Vinit; Sophie Morales
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Paper Abstract

We present a simple and compact phase imaging microscope for long-term observation of non-absorbing biological samples such as unstained cells in nutritive media. The phase image is obtained from a single defocused image taken with a standard wide-field microscope. Using a semi-coherent light source allows us to computationally re-focus image post-acquisition and recover both phase and transmission of the complex specimen. The simplicity of the system reduces both the cost and its physical size and allows a long-term observation of samples directly in a standard biological incubator. The low cost of the system can contribute to the democratization of science by allowing to perform complex long-term biological experiments to the laboratories with constrained budget. In this proceeding we present several results taken with our prototype and discuss the possibilities and limitations of our system.

Paper Details

Date Published: 23 February 2018
PDF: 12 pages
Proc. SPIE 10503, Quantitative Phase Imaging IV, 105031Q (23 February 2018);
Show Author Affiliations
Ondrej Mandula, CEA-LETI Grenoble (France)
Cédric Allier, CEA-LETI Grenoble (France)
Lionel Hervé, CEA-LETI Grenoble (France)
Eric Denarier, Univ. Grenoble Alpes, CEA (France)
Anne Fourest-Lieuvin, Univ. Grenoble Alpes, CEA (France)
Sylvie Gory-Fauré, Univ. Grenoble Alpes, CEA (France)
Angélique Vinit, Univ. Grenoble Alpes, CEA (France)
Sophie Morales, CEA-LETI Grenoble (France)

Published in SPIE Proceedings Vol. 10503:
Quantitative Phase Imaging IV
Gabriel Popescu; YongKeun Park, Editor(s)

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