Share Email Print

Proceedings Paper

Study of bovine pericardium biochemical and biomechanical properties during collagenase degradation using fluorescence lifetime imaging (Conference Presentation)
Author(s): Cai Li; Jeny Shklover; Mojtaba Parvizi; Benjamin E. Sherlock; Alba Alfonso García; Leigh G. Griffiths; Laura Marcu

Paper Abstract

Bovine pericardium (BP) exhibits distinct biochemical and biomechanical properties that are dominant by the structural protein collagen. The enzymatic degradation of collagen molecules is critical for in vivo incorporation and remodeling of BP in tissue engineering applications. A non-destructive method for monitoring BP during degradation would provide a valuable tool for quantifying functional changes initially in vitro and ultimately in vivo. In this study, we demonstrated the sensitivity of multi-spectral fluorescence lifetime imaging system (ms-FLIm) developed by our group to collagen content and compressive modulus of BP during collagenase degradation. A pairwise study was performed using bacterial collagenase to partially digest BP. We measured the biomaterials properties with ms-FLIm and destructive conventional measurements including collagen assay, compressive test and histology. A single factor study design was utilized. Test group samples were digested by bacterial collagenase for 0, 8, 16 and 24 hours, while control group samples were prepared in the Hank’s balanced salt solution to control for time in solution. Statistical analysis was performed using the Kendall τB correlation test. The results demonstrate that fluorescence parameters measured by ms-FLIm are significantly correlated with collagen content and compressive modulus (|τB| > 0.45, p < 0.05). Based on these findings, we aim to predict BP’s collagen content and mechanical properties using fluorescence metrics, and ultimately apply ms-FLIm for non-destructively monitoring of in vivo remodeling of BP.

Paper Details

Date Published: 14 March 2018
Proc. SPIE 10471, Diagnostic and Therapeutic Applications of Light in Cardiology 2018, 1047103 (14 March 2018); doi: 10.1117/12.2287487
Show Author Affiliations
Cai Li, Univ. of California, Davis (United States)
Jeny Shklover, Univ. of California, Davis (United States)
Mojtaba Parvizi, Mayo Clinic (United States)
Benjamin E. Sherlock, Univ. of California, Davis (United States)
Alba Alfonso García, Univ. of California, Davis (United States)
Leigh G. Griffiths, Mayo Clinic (United States)
Laura Marcu, Univ. of California, Davis (United States)

Published in SPIE Proceedings Vol. 10471:
Diagnostic and Therapeutic Applications of Light in Cardiology 2018
Guillermo J. Tearney M.D.; Kenton W. Gregory M.D.; Laura Marcu, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?