Share Email Print
cover

Proceedings Paper

Fluorescence visualization of wound closure in partial and full-thickness wound models (Conference Presentation)

Paper Abstract

Background and Objectives: UV Fluorescence Excitation Imaging (u-FEI) has been shown to be a simple but robust, non-invasive and non-contact method to visualize cells with a high proliferative rate. We had demonstrated the ability of u-FEI to visualize the re-epithelialization of skin wounds in an organ culture system. In this work, we investigated the potential of u-FEI for visualization of wound closure of partial and full-thickness skin wounds. Study Design: Partial and full-thickness skin wounds were created in the tail of rats. Wounds were imaged weekly using u-FEI system operating at 295/340nm excitation/emission wavelengths, which correspond to the excitation/emission bands of the endogenous fluorophore tryptophan. Histology and immunohistology were used to determine the association between fluorescence intensity and proliferation of keratinocyte cells. Results: Similar to human skin, the skin of a rat tail heals by re-epithelialization. Keratinocytes migrated and proliferated from the edge and skin appendages of partial-skin wounds to close the wound by creating neo-epidermis. The fluorescence intensity of the whole wound area increased uniformly during week one and decreased to non-wounded control levels around week three. For full-thickness wounds, keratinocytes migrated only from the wound edges as skin appendages were missing. The fluorescence intensity was higher by the wound edge and marched towards the center during healing. H&E and immunohistology show that changes in fluorescence intensity corresponded to newly formed epidermis. Conclusions: u-FEI of tryptophan allowed visualization of wound closure of partial and full-thickness skin wounds in an in vivo model of wound healing by epithelialization.

Paper Details

Date Published: 14 March 2018
PDF
Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018, 104670P (14 March 2018); doi: 10.1117/12.2287164
Show Author Affiliations
Ying Wang, Massachusetts General Hospital (United States)
Antonio Ortega-Martinez, Massachusetts General Hospital (United States)
William A. Farinelli, Massachusetts General Hospital (United States)
Richard R. Anderson, Massachusetts General Hospital (United States)
Walfre Franco, Massachusetts General Hospital (United States)


Published in SPIE Proceedings Vol. 10467:
Photonics in Dermatology and Plastic Surgery 2018
Bernard Choi; Haishan Zeng, Editor(s)

© SPIE. Terms of Use
Back to Top