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Proceedings Paper

Two-photon FLIM imaging and femtosecond laser nano processing of IPS cells and stem cells (Conference Presentation)
Author(s): Karsten König; Aisada Uchugonova; Hans Georg Breunig; Ana Batista

Paper Abstract

Stem cells and 'induced pluripotent stem cells' (iPS cells) attract considerable attention in medicine. So far, iPS cells which morphologically and functionally resemble embryonic stem cells have been generated by means of viruses through the reprogramming of somatic cells for research purposes. Typically, optical techniques such as light microscopy in combination with fluorescent markers are applied, sometimes accompanied with fluorescence-activated cell sorting (FACS), for the characterization and isolation of both tissue-specific stem and iPS cells. Nowadays, modern laser-based techniques such as nonlinear multiphoton microscopy and optical transfection using femtosecond lasers are used for iPS and stem cell imaging as well as for virus-free optical reprogramming.

Paper Details

Date Published: 14 March 2018
Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104980P (14 March 2018);
Show Author Affiliations
Karsten König, JenLab GmbH (Germany)
Aisada Uchugonova, Univ. des Saarlandes (Germany)
Hans Georg Breunig, JenLab GmbH (Germany)
Ana Batista, JenLab GmbH (Germany)

Published in SPIE Proceedings Vol. 10498:
Multiphoton Microscopy in the Biomedical Sciences XVIII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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