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Proceedings Paper

Automated high resolution full-field spatial coherence tomography for quantitative phase imaging of human red blood cells
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Paper Abstract

We developed an automated high-resolution full-field spatial coherence tomography (FF-SCT) microscope for quantitative phase imaging that is based on the spatial, rather than the temporal, coherence gating. The Red and Green color laser light was used for finding the quantitative phase images of unstained human red blood cells (RBCs). This study uses morphological parameters of unstained RBCs phase images to distinguish between normal and infected cells. We recorded the single interferogram by a FF-SCT microscope for red and green color wavelength and average the two phase images to further reduced the noise artifacts. In order to characterize anemia infected from normal cells different morphological features were extracted and these features were used to train machine learning ensemble model to classify RBCs with high accuracy.

Paper Details

Date Published: 23 February 2018
PDF: 4 pages
Proc. SPIE 10503, Quantitative Phase Imaging IV, 105032I (23 February 2018);
Show Author Affiliations
Neeru Singla, Thapar Institute of Engineering and Technology Univ. (India)
Kavita Dubey, Thapar Institute of Engineering and Technology Univ. (India)
Vishal Srivastava, Thapar Institute of Engineering and Technology Univ. (India)
Azeem Ahmad, Indian Institute of Technology Delhi (India)
D. S. Mehta, Indian Institute of Technology Delhi (India)

Published in SPIE Proceedings Vol. 10503:
Quantitative Phase Imaging IV
Gabriel Popescu; YongKeun Park, Editor(s)

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