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Proceedings Paper

Subcellular analysis of interaction between breast cancer cells and drug by digital holography
Author(s): Jie Zhao; Qiaowen Lin; Dayong Wang; Yunxin Wang; Liting Ouyang; Sha Guo; Qian Yao
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Paper Abstract

Digital holographic microscopy is a promising quantitative phase-contrast imaging technique, which exhibits the advantages of non-destruction, full field of view, quasi-real time, and don’t need dye and external marker to the living biological sample. In this paper, the inverted off-axis image-plane digital holography with pre-magnification is built up to study the living MDA-MB-231 breast cancer cells. The lateral resolution of the proposed experimental setup is 0.87μm, which is verified by the standard USAF test target. Then the system is used to visualize the interaction between living breast cancer cells and drug. The blebbing is observed after the cells are treated by paclitaxel drug, and the distribution of the paclitaxel inside the cells is detected, which is near the cytomembrane, or in other words the end of the microtubules. It will stop the mitosis and cause the death of the cells. It is helpful to reveal the anticancer mechanism of paclitaxel in the subcellular scale.

Paper Details

Date Published: 24 October 2017
PDF: 6 pages
Proc. SPIE 10458, AOPC 2017: 3D Measurement Technology for Intelligent Manufacturing, 104581B (24 October 2017);
Show Author Affiliations
Jie Zhao, Beijing Univ. of Technology (China)
Qiaowen Lin, Beijing Univ. of Technology (China)
Dayong Wang, Beijing Univ. of Technology (China)
Yunxin Wang, Beijing Univ. of Technology (China)
Liting Ouyang, Beijing Univ. of Technology (China)
Sha Guo, Beijing Univ. of Technology (China)
Qian Yao, Beijing Univ. of Technology (China)

Published in SPIE Proceedings Vol. 10458:
AOPC 2017: 3D Measurement Technology for Intelligent Manufacturing
Wolfgang Osten; Anand Krishna Asundi; Huijie Zhao, Editor(s)

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