Share Email Print

Proceedings Paper

Photodynamic inactivation using curcuminoids and Photogem on caenorhabditis elegans
Author(s): Yulli R. Albuquerque; Sebastião Pratavieira; Vanderlei S. Bagnato; Natalia M. Inada; Larissa M. Souza; Ana Afonso; Clovis W. O. de Souza; Kleber T. Oliveira; Fernanda F. Anibal
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Resistance to various anthelmintic drugs is reported in many animals and can become a severe problem for human and animal health. In this study, Photogem® and three curcuminoids compounds (curcumin, demethoxycurcumin, bisdemethoxycurcumin) were used as photosensitizers in the photodynamic inactivation (PDI) in the helminth model Caenorhabditis elegans to investigate the ability of this procedure to worm life cycle. Initially, the presence and location of the photosensitizers in the worm's body were verified by fluorescence confocal microscopy. Curcumin was deposited in the digestive tract and Photogem® along the body of the animal in the incubation time of 12 hours with the photosensitizer. Subsequently, a PDI procedure using a LED device was performed to illuminate the worms treated with the photosensitizers. The worms were observed by optical microscopy until 48 hours after the PDI to verify the changes in motility, the presence of eggs and larvae and the number of live worms. Curcuminoids tested separately and in combination and two light doses of 30 J/m2 no changes were observed in the life cycle of the worm at concentrations of 2 mM and 1 mM. However, in treatment with Photogem® and a light dose of 100 J/m2 a reduction in motility and reproduction of the worm with 0.2 mg/mL was observed after 6 hours of exposure, in addition to the death of most worms at concentrations of 6, 4, and 2 mg/mL. We suggest, therefore, that photodynamic inactivation with Photogem® may present an anthelmintic effect against C. elegans, but there is a need for studies on helminths with parasitic activity.

Paper Details

Date Published: 12 February 2018
PDF: 7 pages
Proc. SPIE 10476, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVII, 1047612 (12 February 2018);
Show Author Affiliations
Yulli R. Albuquerque, Federal Univ. of São Carlos (Brazil)
Sebastião Pratavieira, Univ. de São Paulo (Brazil)
Vanderlei S. Bagnato, Univ. de São Paulo (Brazil)
Natalia M. Inada, Univ. de São Paulo (Brazil)
Larissa M. Souza, Univ. de São Paulo (Brazil)
Ana Afonso, Federal Univ. of São Carlos (Brazil)
Clovis W. O. de Souza, Federal Univ. of São Carlos (Brazil)
Kleber T. Oliveira, Federal Univ. of São Carlos (Brazil)
Fernanda F. Anibal, Federal Univ. of São Carlos (Brazil)

Published in SPIE Proceedings Vol. 10476:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVII
David H. Kessel; Tayyaba Hasan, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?