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Proceedings Paper

Raman spectroscopic studies on exfoliated cells of oral and cervix
Author(s): Arti Hole; Aditi Sahu; Rubina Shaikh; Gunjan Tyagi; C. Murali Krishna
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Paper Abstract

Visual inspection followed by biopsy is the standard procedure for cancer diagnosis. Due to invasive nature of the current diagnostic methods, patients are often non-compliant. Hence, it is necessary to explore less invasive and rapid methods for early detection. Exfoliative cytology is a simple, rapid, and less invasive technique. It is thus well accepted by patients and is suitable for routine applications in population screening programs. Raman spectroscopy (RS) has been increasingly explored for disease diagnosis in the recent past. In vivo RS has previously shown promise in management of both oral and cervix cancers. In vivo applications require on-site instrumentation and stringent experimental conditions. Hence, RS of less invasive samples like exfoliated cells has been explored, as this facilitates collection at multiple screening centers followed by analysis at a centralized facility. In the present study, efficacy of Raman spectroscopy in classification of 15 normal and 29 abnormal oral exfoliated cells specimens and 28 normal and 38 abnormal cervix specimens were explored. Spectra were acquired by Raman microprobe (HE 785, Horiba-Jobin-Yvon, France) from several areas to span the pellet. Spectral acquisition parameters were: microscopic objective: 40X, power: 40 mW, acquisition time: 15 s and average: 3. PCA and PC-LDA of pre-processed spectra was carried out on a 4-model system of normal and tumor of both cervix and oral specimens. Leave-one-out-cross-validation findings indicate ~73 % correct classification. Findings suggest RS of exfoliated cells may serve as a patient-friendly, non-invasive, rapid and objective method for management of cervix and oral cancers.

Paper Details

Date Published: 2 January 2018
PDF: 6 pages
Proc. SPIE 10456, Nanophotonics Australasia 2017, 104562S (2 January 2018); doi: 10.1117/12.2282530
Show Author Affiliations
Arti Hole, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
Aditi Sahu, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
Rubina Shaikh, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
Gunjan Tyagi, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
C. Murali Krishna, Advanced Ctr. for Treatment, Research & Education in Cancer (India)

Published in SPIE Proceedings Vol. 10456:
Nanophotonics Australasia 2017
James W. M. Chon; Baohua Jia, Editor(s)

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