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Proceedings Paper

Large 3D direct laser written scaffolds for tissue engineering applications
Author(s): Anika Trautmann; Marieke Rüth; Horst-Dieter Lemke; Thomas Walther; Ralf Hellmann
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Paper Abstract

We report on the fabrication of three-dimensional direct laser written scaffolds for tissue engineering and the seeding of primary fibroblasts on these structures. Scaffolds are realized by two-photon absorption induced polymerization in the inorganic-organic hybrid polymer OrmoComp using a 515 nm femtosecond laser. A nonstop single-line single-pass writing process is implemented in order to produce periodic reproducible large scaled structures with a dimension in the range of several millimeters and reduce process time to less than one hour. This method allows us to determine optimized process parameters for writing stable structures while achieving pore sizes ranging from 5 μm to 90 μm and a scanning speed of up to 5 mm/s. After a multi-stage post-treatment, normal human dermal fibroblasts are applied to the scaffolds to test if these macroscopic structures with large surface and numerous small gaps between the pores provide nontoxic conditions. Furthermore, we study the cell behavior in this environment and observe both cell growth on as well as ingrowth on the three-dimensional structures. In particular, fibroblasts adhere and grow also on the vertical walls of the scaffolds.

Paper Details

Date Published: 2 January 2018
PDF: 6 pages
Proc. SPIE 10456, Nanophotonics Australasia 2017, 104565A (2 January 2018); doi: 10.1117/12.2282417
Show Author Affiliations
Anika Trautmann, Univ. of Applied Sciences Aschaffenburg (Germany)
Marieke Rüth, eXcorLab GmbH (Germany)
Horst-Dieter Lemke, eXcorLab GmbH (Germany)
Thomas Walther, Technische Univ. Darmstadt (Germany)
Ralf Hellmann, Univ. of Applied Sciences Aschaffenburg (Germany)

Published in SPIE Proceedings Vol. 10456:
Nanophotonics Australasia 2017
James W. M. Chon; Baohua Jia, Editor(s)

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