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Proceedings Paper

Gaussian vs. Bessel light-sheets: performance analysis in live large sample imaging
Author(s): Sascha L. Reidt; Ricardo B. C. Correia; Mark Donnachie; Cornelis J. Weijer; Michael P. MacDonald
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Paper Abstract

Lightsheet fluorescence microscopy (LSFM) has rapidly progressed in the past decade from an emerging technology into an established methodology. This progress has largely been driven by its suitability to developmental biology, where it is able to give excellent spatial-temporal resolution over relatively large fields of view with good contrast and low phototoxicity. In many respects it is superseding confocal microscopy. However, it is no magic bullet and still struggles to image deeply in more highly scattering samples. Many solutions to this challenge have been presented, including, Airy and Bessel illumination, 2-photon operation and deconvolution techniques. In this work, we show a comparison between a simple but effective Gaussian beam illumination and Bessel illumination for imaging in chicken embryos. Whilst Bessel illumination is shown to be of benefit when a greater depth of field is required, it is not possible to see any benefits for imaging into the highly scattering tissue of the chick embryo.

Paper Details

Date Published: 25 August 2017
PDF: 10 pages
Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 1034709 (25 August 2017); doi: 10.1117/12.2277324
Show Author Affiliations
Sascha L. Reidt, Univ. of Dundee (United Kingdom)
Ricardo B. C. Correia, Univ. of Dundee (United Kingdom)
Mark Donnachie, Univ. of Dundee (United Kingdom)
Cornelis J. Weijer, Univ. of Dundee (United Kingdom)
Michael P. MacDonald, Univ. of Dundee (United Kingdom)

Published in SPIE Proceedings Vol. 10347:
Optical Trapping and Optical Micromanipulation XIV
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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