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Proceedings Paper

Manipulating mutant bacterial cells by tug-of-war multi-trap tweezers (Conference Presentation)
Author(s): Josh Lamstein; Anna S. Bezryadina; Daryl Preece; Joseph C. Chen; Zhigang Chen

Paper Abstract

We design and demonstrate multi-trap tug-of-war (TOW) optical tweezers with object-adapted optical potentials for trapping and manipulating asymmetric particles and biological samples such as mutant bacterial cells. While dual TOW tweezers can effectively trap rod-shaped objects and even stretch them laterally, triangular TOW tweezers enable in-plane trapping of larger asymmetric objects which do not necessarily have mirror symmetry. When trapping with the dual TOW tweezers, we previously demonstrated that they are more stable than Gaussian beam-based dual traps, and the strong lateral pulling forces from the TOW optical tweezers can stretch and even break apart cellular clusters. Here we show multi-trap TOW (with 3 and 4 arms) optical tweezers can be employed to control and manipulate mutant Sinorhizobium meliloti bacterial cells, which are typically multi-pronged. We discuss the advantage of such TOW beam-based optical tweezers over traditional Gaussian beam-based holographic tweezers, and the potential applications of these TOW tweezers in studying cellular viscoelasticity, biomechanics, motility, and intercellular interactions.

Paper Details

Date Published: 11 October 2017
Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 1034705 (11 October 2017); doi: 10.1117/12.2277107
Show Author Affiliations
Josh Lamstein, San Francisco State Univ. (United States)
Anna S. Bezryadina, Univ. of California, San Diego (United States)
San Francisco State Univ. (United States)
Daryl Preece, Univ. of California, San Diego (United States)
Joseph C. Chen, San Francisco State Univ. (United States)
Zhigang Chen, San Francisco State Univ. (United States)
Nankai Univ. (China)

Published in SPIE Proceedings Vol. 10347:
Optical Trapping and Optical Micromanipulation XIV
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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