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Proceedings Paper

Progress on an optical trapping assay to measure DNA folding pathways in sperm
Author(s): Luka M. Devenica; Bishop Grimm; Terri-Anne Hultum; Ashley R. Carter
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Paper Abstract

DNA undergoes a dramatic condensation in sperm nuclei. During this condensation, the DNA rapidly folds into a series of toroids when protamine proteins replace histone proteins. Measuring the mechanics and folding pathway for this incredible condensation is an important goal. Here, we report on progress to use an in vitro, optical trapping assay to measure the DNA folding dynamics for this process. In this assay, a single DNA molecule with its associated histone proteins is attached to a cover slip and to an optically trapped bead. Movement of the optical trap applies a force on the bead, stretching the DNA to a particular extension. When protamine is added, the extension changes, allowing us to measure the preliminary folding dynamics for the process.

Paper Details

Date Published: 25 August 2017
PDF: 12 pages
Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 103472V (25 August 2017); doi: 10.1117/12.2275722
Show Author Affiliations
Luka M. Devenica, Amherst College (United States)
Bishop Grimm, Amherst College (United States)
Terri-Anne Hultum, Amherst College (United States)
Ashley R. Carter, Amherst College (United States)

Published in SPIE Proceedings Vol. 10347:
Optical Trapping and Optical Micromanipulation XIV
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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