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Proceedings Paper

Adaptive optical imaging through complex living plant cells
Author(s): Yosuke Tamada; Yutaka Hayano; Takashi Murata; Shin Oya; Yusuke Honma; Minoru Kanazawa; Noriaki Miura; Mitsuyasu Hasebe; Yasuhiro Kamei; Masayuki Hattori
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Paper Abstract

Live-cell imaging using fluorescent molecules is now essential for biological researches. However, images of living cells are accompanied with blur, which becomes stronger according to the depth inside the cells and tissues. This image blur is caused by the disturbance on light that goes through optically inhomogeneous living cells and tissues. Here, we show adaptive optics (AO) imaging of living plant cells. AO has been developed in astronomy to correct the disturbance on light caused by atmospheric turbulence. We developed AO microscope effective for the observation of living plant cells with strong disturbance by chloroplasts, and successfully obtained clear images inside plant cells.

Paper Details

Date Published: 18 April 2017
PDF: 3 pages
Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510K (18 April 2017); doi: 10.1117/12.2275419
Show Author Affiliations
Yosuke Tamada, National Institute for Basic Biology (Japan)
Graduate Univ. for Advanced Studies (Japan)
Yutaka Hayano, National Astronomical Observatory of Japan (Japan)
Graduate Univ. for Advanced Studies (Japan)
Takashi Murata, National Institute for Basic Biology (Japan)
Graduate Univ. for Advanced Studies (Japan)
Shin Oya, National Astronomical Observatory of Japan (Japan)
Graduate Univ. for Advanced Studies (Japan)
Yusuke Honma, Kitami Institute of Technology (Japan)
Minoru Kanazawa, Kitami Institute of Technology (Japan)
Noriaki Miura, Kitami Institute of Technology (Japan)
Mitsuyasu Hasebe, National Institute for Basic Biology (Japan)
Graduate Univ. for Advanced Studies (Japan)
Yasuhiro Kamei, National Institute for Basic Biology (Japan)
Graduate Univ. for Advanced Studies (Japan)
Masayuki Hattori, National Institute for Basic Biology (Japan)


Published in SPIE Proceedings Vol. 10251:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji, Editor(s)

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