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Proceedings Paper

Microscopic video observation of capillary vessel systems using diffuse back lighting
Author(s): Minako Sakai; Hiroki Arai; Toshiaki Iwai
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Paper Abstract

We have been developing a simple and practical video microscopy system based on absorption spectra of biological substance to perform spectroscopic observation of living tissues. The diffuse backlighting effect is actively used in the developed system, which is generated by multiple light scattering in the tissue. It is demonstrated that the light specularly reflected from the skin surface can be completely suppressed in the microscopic observation and the biological activity of the capillary vessel systems distributed under the skin can be successfully observed. As a result, we can confirm the effectiveness of the video microscopy system using diffuse backlighting and the applicability of our developed system.

Paper Details

Date Published: 18 April 2017
PDF: 3 pages
Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510E (18 April 2017); doi: 10.1117/12.2274907
Show Author Affiliations
Minako Sakai, Tokyo Univ. of Agriculture and Technology (Japan)
Hiroki Arai, Tokyo Univ. of Agriculture and Technology (Japan)
Toshiaki Iwai, Tokyo Univ. of Agriculture and Technology (Japan)

Published in SPIE Proceedings Vol. 10251:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji, Editor(s)

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