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Proceedings Paper

Gamma globulins-induced interaction between two red blood cells: forces measurement with optical tweezers
Author(s): Kisung Lee; Alexei Muravyov; Alexei Semenov; Christian Wagner; Alexander Priezzhev; Eugeny Lyubin; Andrey Fedyanin
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Paper Abstract

The protein contribution to the red blood cell (RBC) aggregation is studied using the in-house made two-channeled optical tweezers. The cells interaction was characterized using two forces: the force required for separating two cells (FD – disaggregating force) and the force required for holding them from their spontaneous aggregation (FA – aggregating force). The gamma globulin solutions with/without albumin were used to induce the RBC aggregation. The strong interaction (3-10 pN) between the cells was measured within the contact formed using optical tweezers. We found that FD becomes stronger as the gamma globulin concentration increases, while the addition of albumin to the solution led to the significant (few fold) enhancement of the cells interaction forces. However, despite of the strong interaction between the cells their spontaneous overlapping was not observed, unlike the case in plasma, where the cells did increase their overlapping surface, when attached with small interacting surface and released from optical traps. This work in addition to our previous work with model solutions of fibrinogen allows us to conclude that the synergy of blood components is one of the most important features that contribute to the reversible RBC aggregation.

Paper Details

Date Published: 24 March 2017
PDF: 5 pages
Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 1033606 (24 March 2017); doi: 10.1117/12.2271646
Show Author Affiliations
Kisung Lee, Saarland Univ. (Germany)
Lomonosov Moscow State Univ. (Russian Federation)
Alexei Muravyov, Yaroslavl State Pedagogical Univ. (Russian Federation)
Alexei Semenov, Lomonosov Moscow State Univ. (Russian Federation)
Christian Wagner, Saarland Univ. (Germany)
Univ. of Luxembourg (Luxembourg)
Alexander Priezzhev, Lomonosov Moscow State Univ. (Russian Federation)
Eugeny Lyubin, Lomonosov Moscow State Univ. (Russian Federation)
Andrey Fedyanin, Lomonosov Moscow State Univ. (Russian Federation)

Published in SPIE Proceedings Vol. 10336:
Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII
Valery V. Tuchin; Elina A. Genina; Dmitry E. Postnov; Vladimir L. Derbov, Editor(s)

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