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Proceedings Paper

Extended depth-of-field microscopy with a high-speed deformable mirror
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Paper Abstract

Wide-field fluorescence microscopy is generally limited to either small volumes or low temporal resolution. We present a microscope add-on that provides fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror of update rate 20kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

Paper Details

Date Published: 21 February 2017
PDF: 7 pages
Proc. SPIE 10073, Adaptive Optics and Wavefront Control for Biological Systems III, 100730E (21 February 2017); doi: 10.1117/12.2271502
Show Author Affiliations
William J. Shain, Boston Univ. (United States)
Nicholas A. Vickers, Boston Univ. (United States)
Bennett B. Goldberg, Boston Univ. (United States)
Northwestern Univ. (United States)
Thomas Bifano, Boston Univ. (United States)
Jerome Mertz, Boston Univ. (United States)


Published in SPIE Proceedings Vol. 10073:
Adaptive Optics and Wavefront Control for Biological Systems III
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

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